LipoJet?轉染試劑盒(Ver. II)

產品介紹：
利用我們的創新和專有的脂質體共軛合成技術，LipoJet™轉染試劑盒，運用了新型的含氟陽離子脂質體配方，同市場上的其他脂質體轉染試劑有著顯著 的區別，其特點是轉染效率高、毒性小。LipoJet™轉染試劑盒是最強大的基因 轉導工具，適于幾乎有所有體外基因轉導的應用，包括質粒DNA、siRNA和shRNA轉染和DNA/siRNA共轉等。

試劑盒內容：
- LipoJet™ 試劑，1.0ml，足夠用1000次DNA的轉染(24孔板中0.5 ug DNA/孔)足夠用1000次siRNA轉染(24孔板中10 nM siRNA/孔)。
- LipoJet™轉染緩沖液(5X )，按照最大化的轉染效率配制，8.0ml濃縮液就可以制成40ml的工作液體。

產品特點：
- 適用于廣泛的哺乳動物細胞
- 極高效率地轉染DNA，siRNA和DNA/siRNA共轉染
- 10 nM siRNA能達到95%的沉默效應
- 無血清和抗生素干擾
- 簡單易用的操作步驟
- 適用于高通量的應用
- 細胞毒性最低

儲存條件 ：
4⁰C儲存。若儲藏合適，產品的穩定性能保持12個月以上。

LipoJet™體外轉染試劑具有極廣譜的轉染活性。

以下圖例表明LipoJet™體外轉染試劑盒出色的DNA和siRNA轉染效率 。

A efficiency comparison of LipoJet™ reagent vs. brand name products to transfect Hela, Cos-7, NIH-3T3, CHO and HEK293 (left panel) and Cos-7 (right panel).  pEGFP-N3 cDNA (0.5 µg/well of 24-well plate) was transfected into different mammalian cells per the standard transfection protocols in presence of serum (10% FBS) and antibiotics as recommended by manufacturers.  The transfection efficiency were analyzed via FACS 48 hours post transfection.


A toxicity comparison of LipoJet™ reagent vs. brand name products to transfect Hela cells.  pEGFP-N3 cDNA (1.0 µg/well of 24-well plate) was transfected to Hela cells per the standard transfection protocols in presence of serum (10% FBS) and antibiotics as recommended by manufacturers.  The MTT assay (right panel) and phase contrast imaging (left panel) were used to analyze the cell viability 48 hours post transfection.


A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on HEK293T cell. pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well and 0.5 µg/per well of 24-well plate) was transfected into 293T cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA (µl/µg) ratio=3) and PolyJet™ (lower panel at at PolyJet™/DNA (µl/µg) ratio=3) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.


A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on Hela cell.  pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well and 0.5 µg/per well of 24-well plate) was transfected into Hela cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA (µl/µg) ratio=3) and PolyJet™ (lower panel at at PolyJet™/DNA (µl/µg) ratio=3) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.




LipoJet™試劑介導的DNA/siRNA共轉染表現出色的基因沉默效應。分別由LipoJet™試劑轉染GFP的cDNA (左圖, 0.25µg/孔，24孔板)作為對照和共轉染GFP的cDNA (0.25µg/孔，24孔板)/GFP的siRNA (終濃度10 nM，右圖)至HEK293細胞 (上圖), Hela細胞(中圖)和SaoS-2細胞(下圖)。轉染24小時后，使用尼康Eclipse熒光顯微鏡檢查GFP熒光。結果顯示，GFP的cDNA和GFP的siRNA共轉染導致GFP的表達被顯著抑制。


Exceptional DNA/siRNA co-transfection efficiency on HUVEC. Co-transfection of mCherry cDNA (0.10 µg per well of 24-well plate) and FITC conjugated siRNA (final 30 nM per well of 24-well plate) to HUVEC with LipoJet™ reagent gave rise to 60% mCherry+ (phase contrast overlapped with mCherry imaging, left panel) and nearly 100% FITC-siRNA+ (phase contrast overlapped with FITC imaging, right panel) HUVEC 24 hours after transfection.  The pictures were given from Dr. Pan Kong of USC as courtesy. 



Excellent silencing of endogenously expressed KIF11 (also known as EG5) in HEK293 (upper panel) and Hela (lower panel) cells with LipoJet™ reagent at 10 nM EG5 siRNA. KIF11 (also known as EG5) encodes a motor protein that belongs to the kinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. LipoJet™ reagent effectively delivers EG5 siRNA (final 10 nM) to HEK293 and Hela cells, leading to more than 80% of "round-up" phenotype of HEK293 and Hela cells 24h post transfection over negative control (final 10 nM with sham EG5 siRNA). The phenotype of "rounded-up" HEK293 and Hela cells were visualized 24h post transfection with a Nikon microscope.



LipoJet™轉染試劑介導的基因沉默可顯著地抑制HEK293細胞(上圖)和Hela(下圖)內源型EG5的表達。KIF11 (也稱為EG5)編碼了一個啟動蛋白，這啟動蛋白屬于驅動蛋白家族，參與染色體定位和細胞有絲分裂時兩端的紡錘體的形成。EG5水平降低會阻礙有絲分裂。LipoJet™試劑能有效的將EG5 siRNA(終濃度10 nM)導入到HEK293和Hela細胞中。轉染24小時后，參照陰性對照(終濃度是10 nM+假EG5 siRNA)，LipoJet™介導的EG5 siRNA的導入顯著地抑制了細胞有絲分裂，導致了大于80%的細胞出現圓頂表型。

轉染試劑操作步驟及其使用指南 ： 亚洲综合无码一区二区三区不卡,亚洲情A成黄在线观看动漫尤物,亚洲色自偷自拍另类小说,国产A片
- LipoJet™試劑DNA和siRNA轉染操作步驟
- 簡易DNA轉染操作步驟(適合熟練操作者 
- 簡易siRNA轉染操作步驟(適合熟練操作者) 
- LipoJet™試劑DNA/siRNA共轉染操作步驟 
- 注意事項及轉染竅門 

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用戶評價：

I tried the plasmid/siRNA co-transfection using the LipoJet sample in EAhy926 cell which is a HUVEC cell line. The results are pretty good, at least for the transfection efficiency. For the plasmid transfection, 48 hours later, around 60% cell are transduced. For siRNA, the efficiency is almost 100%. We are ordering more LipoJet reagent to use from now.

-----Dr. Pan Kong, University of Southern California

We got >90% efficiency with LipoJet vs. 80% with X-tremeGENE 9 on 293T cell. Definitely will order more....

-----Dr. Nuo Yang from Roswell Park Cancer Institute

Here are the results from our preliminary experiments with LipoJet. We are very satisfied with its efficiency. Unfortunately, we were out of Fugene 6/HD and were unable to test them side by side, but based on previous experience, we do believe that your product worked with better efficiency.

------Dr. Alison McKelvey from University of Pittsburgh

I am happy to say that I had great success with your transfection reagents on OKF6/TERT2 human keratinocytes. I will put together the data once I have completed my analysis. I really like the LipoJet. My cell of interest did not do very well in the GenMute although my control cells did. We want to go ahead with ordering ASAP. I also have a coupon for 10% for a PO order.

-----Dr. Michelle Simpson-Abelson from UPMC

We did indeed test them side by side with our homemade CaPhos. The results are great,  LipoJet and CalFectin both did extremely well. LipoJet being the better of the two. I will send you the results when  I get them,  I am currently out of the lab but we will be ordering more for certain. Thank you for sending those samples.

------Dr. Ahmed Hassib, Cornell University

For the LipoJet, I only compared it with Lipo LTX in Hela cells. The LipoJet gave a much higher efficiency (~35% better than Lipo LTX) 24 hours after transfection. We will order some LipoJet.

-----A beta tester from USC