Contents Per Kit:
●
1x1.0 ml of GenJet?
DNA Transfection Reagent
● 1x1.0 ml (x50) of HapiCell? Transfection Toxicity Removal Cocktail
Description
GenJet? DNA In Vitro
Tranfection Reagent for SMC is pre-optimized for transfecting smooth muscle
cells. Smooth muscle cells are notoriously hard to transfect partly due to
the transfection toxicity related cell death. In combination
of our proprietary HapiCell? Toxicity
Removal Cocktail, the pre-optimized transfection
reagent gave up to 75% efficiency.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on Hela cells. GenJet? reagent, 1.0 ml, is sufficient for 300 to
600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
|
Summary of
Optimal Transfection Conditions:
Confluence on the
day of transfection
Cell culture
conditions
GenJet? (μl) : DNA (μg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet?/DNA
Complex
Presence of Serum/Antibiotics
during Transfection
Change Medium After Transfection
Treatment with HapiCell? Toxicity Removal
Cocktail
Maximal Efficiency
Transfection
Results:
Reporter Gene
Plasmid
Efficiency (GFP %) |
95%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
Yes, ~5 Hours After Transfection Followed by PBS Wash
48 hours
EGFP
pEGFP-N3 (CMV promoter)
55% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
