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    2. GenJet? In Vitro DNA Tranfection Kit for SMC

      價格: ¥1,680.00

      購買數量:

      • 目錄號: SL100489-SMC
      • 包裝: 1 kit (2x1.0 ml)

      請選擇:



      Contents Per Kit:
      1x1.0 ml of GenJet? DNA Transfection Reagent
      ● 1x1.0 ml (x50) of HapiCell? Transfection Toxicity Removal Cocktail

      Description
      GenJet? DNA In Vitro Tranfection Reagent for SMC is pre-optimized for transfecting smooth muscle cells. Smooth muscle cells are notoriously hard to transfect partly due to the transfection toxicity related cell death. In combination of our proprietary HapiCell? Toxicity Removal Cocktail, the pre-optimized transfection reagent gave up to 75% efficiency.

      Refer to the following optimal transfection conditions for maximal transfection efficiency on Hela c
      ells. GenJet? reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

      Summary of Optimal Transfection Conditions:
      Confluence on the day of transfection
      Cell culture conditions
      GenJet? (μl) : DNA (μg) Ratio
      Diluent for DNA and Transfection Reagent

      Incubation Time to Form GenJet?/DNA Complex
      Presence of Serum/Antibiotics during Transfection
      Change Medium After Transfection
      Treatment with HapiCell? Toxicity Removal Cocktail
      Maximal Efficiency

      Transfection Results:
      Reporter Gene
      Plasmid

      Efficiency (GFP %)


      95%
      DMEM with 4.5 g/L glucose, 10% FBS

      3:1
      Serum-free DMEM with 4.5 g/L glucose

      15 minutes at RT
      Yes
      Yes, ~5 Hours After Transfection
      Yes, ~5 Hours After Transfection Followed by PBS Wash
      48 hours


      EGFP
      pEGFP-N3 (CMV promoter)

      55%

      Storage Condition
      Store at 4 °C. If stored properly, the product is stable for 12 months or longer

      Data Sheet




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